After three washes, the membrane was next incubated 1 h at room temperature with goat anti-rabbit or goat anti-mouse secondary antibodies (1:20,000) (Calbiochem, Beeston Nottingham, UK) conjugated to horseradish peroxydase
After three washes, the membrane was next incubated 1 h at room temperature with goat anti-rabbit or goat anti-mouse secondary antibodies (1:20,000) (Calbiochem, Beeston Nottingham, UK) conjugated to horseradish peroxydase.…