The exact proteolytic sites for lots of the known protein substrates have not been validated yet for the reasons that 1) ectodomain shedding occurs in the immediate extracellular juxtamembrane region, which is also where O-glycosylation is often found; 2) multiple cleavages occur on the same stalk due to the low specificity; and 3) the involvement of additional proteases, like additional ADAMs family proteases and peptidases
The exact proteolytic sites for lots of the known protein substrates have not been validated yet for the reasons that 1) ectodomain shedding occurs in the immediate extracellular juxtamembrane region,…