Hasta serumlar? ile fare beyin dokusunun inkbasyonu sonucunda hem parankimal hem de vaskler yap?lara kar?? ?zgl immnreaktivite tespit edilmi?tir

Hasta serumlar? ile fare beyin dokusunun inkbasyonu sonucunda hem parankimal hem de vaskler yap?lara kar?? ?zgl immnreaktivite tespit edilmi?tir. whereas just two of control sera demonstrated no significant parenchymal vascular immunoreactivity (p=.025). Furthermore to vascular immunoreactivity, reticular and filamentous immunopositive structures were discovered in brain parts of 5 away of 10 sufferers. No such immunoreactivity was discovered in areas incubated with control sera (p=.016). Bottom line We detected a particular immunoreactivity against parenchymal and vascular filamentous buildings in neuro-Beh?et sufferers sera. Humoral autoimmunity might are likely involved in the pathogenesis of neuro-Beh?ets disease furthermore to cellular defense response. Results of the primary research will end up being examined with a lot of handles and sufferers, to determine whether it’s the reason or the full total result and, further research are underway to reveal the type of epitope to that your immunoreactivity was directed against also to create a diagnostic lab method for looking into central nervous program participation in Beh?ets sufferers. strong course=”kwd-title” Keywords: Beh?ets Disease, Neuro-Beh?ets Disease, Autoimmunity, Immunohistochemistry ?ZET Ama? Beh?et Hastal??? ?oklu sistem tutulumu ile giden, etiyolojisi ve fizyopatolojisi bilinmeyen, kronik, inflamatuar bir hastal?kt?r. Hastal???n tan?s?n?klinik bulgulara dayanan kriterlere g n?re konulmas? ve ?zellikle di?er bulgular?n e?lik etmedi?we, n?rolojik tutulumla ba?layan hastalarda tan?n?belirlenmesindeki g n?lkler, ?zgl bir laboratuvar testinin eksikli?ine dikkati ?ekmektedir. Y?ntem Bu ?al??mada, immn sistem bozuklu?una ba?l? geli?ti?we d?nlen durumlarda uygulanan, indirekt immnfloresan boyama tekni?we kullanarak, SSS tutulumu olan Beh?et hastalar?n?n serumlar?nda, SSSye kar?? otoantikor varl??? ara?t?r?lm??t?r. ?nce hasta ve kontrol serumlar?, ?zgl olmayan boyanmay? ?nleyebilmek we?in kobay karaci?eri ile preabsorbsiyona tabi tutulmu?lard?r. Takiben fare beyin dokusu ile inkbe edilerek, floresan (FITC) ile konjuge, insan IgGsine kar?? ke?ide haz?rlanm?? IgG ile i?aretlenmi?tir. Beyin kesitleri floresan mikroskop ile de?erlendirilmi?tir. Bulgular On n?ro-Beh?et hastas? ve 10 ya? uyumlu kontrol bireyin serumlar? immnreaktivite a??s?ndan incelenmi?tir. Hasta serumlar? ile fare Papain Inhibitor beyin dokusunun inkbasyonu sonucunda hem parankimal hem de vaskler yap?lara kar?? ?zgl immnreaktivite tespit edilmi?tir. 10 hastan?8inde parankimal vaskler boyanma varken n, kontrollerin sadece 2 tanesinde hastalarla Papain Inhibitor kar??la?t?r?ld???nda ?okay belirgin olmayan immnreaktivite g?rld (p=0,025). Vaskler immnreaktiviteye ek olarak, 10 hastan?5inde filament n?z ve retikler yap?larda immnreaktivite g?zlenirken, kontrollerin serumlar?yla yap?lan inkbasyonlarda, hello there?birinde benzer bir boyanma saptanmam??t?r (p=0,016). Sonu? SSS tutulumu olan Beh?et hastalar?n?n serumlar?nda, hem parankimal hem de vaskler yap?lara kar?? ?zgl immnreaktivite tespit edilmi?tir. Bu bulgular, Beh?et hastal??? SSS patogenezinde hmoral otoimmnitenin rol olabilece?ini d?ndrmektedir. Daha ?okay state?da hasta ve kontroln dahil edilece?we bir ?al??ma ile, bu ?n ?al??mada tespit edilen bulgular?n, bir sebep mi yoksa sonu? mu oldu?u ara?t?r?lacakt?r. Bu tespit edilen ?zgn immnoreaktivitenin ne tr bir epitopa ait oldu?u incelenerek, Beh?et Hastal???n?n SSS tutulumu i?in klinikte kullan?labilecek bir laboratuvar con?ntemi geli?tirilmeye ?al???lacakt?r. Launch Beh?ets disease is a systemic inflammatory disease charecterized with recurrent oral-genital Mouse monoclonal to COX4I1 afthous eyes and ulcers involvement. Multi-system involvement may be noticed; the skin, joint parts, eye, central anxious system (CNS) and gastrointestinal system (GIS) are involved most commonly. It may affect small, moderate and large arteries and veins (1). Although the etiology is not known exactly, it is thought to be an autoimmune disease brought on by environmental factors in individuals with genetic predisposition (2). Although the disease may be observed at any age, it frequently occurs at the age of 20C35 years. In men and in individuals with an early age of onset, severe complications and mortality are observed more commonly (3,4,5). The frequency of neurological involvement in Beh?ets disease ranges between 2.2% and 47% (6,7,8). In one study, CNS involvement was observed with a rate of 34% in autopsy series in 170 patients who were diagnosed with neuro-Beh?ets with a rate of 10% while living. This variability is usually propably related not only with ethnic and geographical difference, but also with how neurological involvement is usually defined. Headache which is not questioned in detail may lead to confusion in terms of the frequency of neurological involvement (9). In a prospective study conducted in Turkey, neurological involvement was found with a rate of 5.3% similar to many studies (10,11,12). In studies directed to investigate the etiology Papain Inhibitor and pathogenesis of Beh?ets disease, the effect of different factors including genetic predisposition, infectious brokers, heat schock proteins, humoral and cellular immunity changes, endothelial dysfunction and disorders in.