ELISA showed a better overall performance than ICT. their employment as screening checks. In conclusion, the combination of easy-to-perform checks, such as ICT and ELISA, could improve level of sensitivity in the serodiagnosis of Mediterranean VL. complex [1]. causes VL in Europe, where the disease is definitely hypoendemic in nine countries; nearly 75% of the instances in the World Health Corporation (WHO) European region happen in Albania, Georgia, Italy, and Spain [2]. Several VL outbreaks have occurred in the WHO Western region since 2009, including in Chrysin Madrid in Spain [3], in Bologna and Modena in northeastern Italy Chrysin [4,5], and in Tbilisi in Georgia [6]. The analysis of VL is definitely difficult; efforts have been made to replace the microscopic recognition of parasites in bone marrow specimens with more sensitive molecular methods [7,8]. However, despite the amount of evidence concerning the optimal overall performance of PCR assays, such techniques are restricted to well-equipped laboratories, and standardization of protocols is definitely lacking [9]. Besides parasite detection, serological tools provide good diagnostic accuracy [9,10]. Different serological checks are currently available and widely used for analysis, including the quick immunochromatographic test based on the recombinant protein K39 (rK39-ICT), the indirect fluorescent antibody test (IFAT), the enzyme-linked immunosorbent assay (ELISA), the western blot (WB), and the direct agglutination test [8,9,10]. Even though VL is definitely common in Mediterranean Europe, serological checks have been primarily validated in highly endemic areas, including the Horn of Africa, the Indian subcontinent, and Brazil [11]. The aim of this study was to evaluate and compare the overall performance of seven serological checks in the analysis of autochthonous VL from northeastern Italy, by carrying out a retrospective analysis of sera collected from VL and non-VL individuals. 2. Materials and Methods This study was designed TCF7L3 like a retrospective comparative study. We obtained samples from individuals with suspected VL that were admitted to healthcare facilities in the Emilia-Romagna region Chrysin (northeastern Italy). Among samples submitted for VL analysis in the Regional Research Centre for Microbiological Emergencies (CRREM), Unit of Microbiology, St. Orsola-Malpighi University or college Hospital, Bologna (Italy) between 2013 and 2015, 77 serum samples were selected; VL was confirmed in 27 instances and dismissed in 50 instances. Clinical indications suggestive of VL included long term fever, splenomegaly, and loss of excess weight, while laboratory data included anemia, thrombocytopenia, leukopenia, and/or hypergammaglobulinemia [1]. VL analysis was confirmed by PCR analysis; parasitic DNA was amplified in peripheral blood and/or bone marrow aspirates by employing simultaneously two real-time PCR assays as explained [12]. The protocol of this study was authorized by the Ethics Committee of the St. Orsola-Malpighi University Hospital (prot. n.1049/2016). Index checks included the following seven serological assays: IFAT: Leishmania-Spot IF, BioMrieux (Marcy-ltoile, France); rK39-ICT: (i) Leishmania Dipstick Rapydtest, Apacor (Wokingham, England), (ii) Chrysin On Site Leishmania IgG/IgM Combo Quick Test, CTK Biotech (Poway, CA, USA), (iii) LEISHMANIA Strip quick Test, Cypress Diagnostics (Hulshout, Belgium); ELISA: (i) Leishmania ELISA IgG + IgM, Vircell Microbiologists (Granada, Spain); (ii) Leishmania infantum IgG ELISA, NovaTec (Dietzenbach, Germany); WB: Leishmania European BLOT IgG, LDBio Diagnostics (Lyon, France) [13]. All checks were performed according to the manufacturers instructions. By means of true positive, true negative, false positive and false bad rates, we computed level of sensitivity, specificity, and accuracy. Statistical analysis was performed by using SPSS v. 20.0 (IBM Corp., Armonk, NY, USA). 3. Results Seventy-seven serum samples from individuals with suspected VL were selected, of which 27 were from individuals with confirmed VL and 50 were from VL bad individuals. The mean age of individuals was 44 years (range: one month to 87 years). Fifteen individuals were children (range: one month to 13 years), 11 individuals (14%) were HIV-positive, and one individual was immunocompromised with hematological malignancy. The overall level of sensitivity, specificity, and accuracy Chrysin of the index checks are summarized in Table 1. ELISA showed a better overall performance than ICT. In addition, IFAT and WB showed the best level of sensitivity, both having a value of 96%. All checks except WB exhibited high specificity ideals. By restricting results to immunocompetent individuals (= 65), the overall level of sensitivity of the checks increased significantly; the level of sensitivity range for rK39-ICT was 59C77%, the level of sensitivity range for ELISA was 68C82%, while IFAT and WB reached a level of sensitivity of 100%. No significant difference was observed for specificity ideals (data not demonstrated). Table 1 Overall performance of seven serological checks for visceral leishmaniasis.
ELISA showed a better overall performance than ICT
- Post author:abic2004
- Post published:October 13, 2024
- Post category:mGlu Group I Receptors