Therefore organ pieces of approximately 1?g or cloacal swabs, respectively, were added to 9?mL of buffered peptone water and incubated at 38. as the course of their fecal RIP2 kinase inhibitor 2 excretion was observed. Results Antibody production was hardly detectable after the 1st vaccination but improved after booster vaccinations. Both the Enteritidis and RIP2 kinase inhibitor 2 the Typhimurium vaccine strain were reisolated from caecum material and organ samples. After booster vaccinations the re-isolation rates were reduced. The dropping of the vaccine strains was most pronounced after the 1st vaccination. Electronic supplementary material The online version of this article (10.1186/s13104-018-3462-y) contains supplementary material, which is available to authorized users. Keywords: are often subclinical in poultry. Like a zoonotic agent however present a serious danger to general public health. The bacteria may be launched into the food RIP2 kinase inhibitor 2 chain and cause enteritis in humans. In people at risk such as babies, small children, and the elderly, infections may be serious. Therefore the primary aim of control in poultry is to reduce the contamination of poultry products and consequently the transmission to humans. At the same time very young animals poults might profit from the safety against Typhimurium (ST) which may cause severe disease with great economic losses in the early stage of existence. Vaccination of livestock as a tool to reduce human being has been researched for decades now. However, not much work has been dedicated to the vaccination of turkeys [1]. Krger et al. [2] tested a live vaccine for Enteritidis (SE) in turkeys but found it unsuitable for reduction of dropping or prevention of systemic spread of virulent SE. Different types of killed vaccines have been reported to reduce dropping of SE and internal organ colonization of SE in turkeys, and to confer safety that is passed on to the progeny of vaccinated breeders (examined by [3]). Thain et al. [4] reported high IgG anti body titers after vaccination of turkey breeders and high levels of maternal antibodies in their offspring. Tenk et al. [5] observed low serum antibody titers after vaccination of turkeys but better overall performance. Overall however, it has been argued that live vaccines are better suited to stimulate cellular immunity and to confer safety in poultry [6]. For turkeys more information on the use of live vaccines is needed. Recently, two studies by our group tackled the use of a combined Enteritidis (SE)- and Typhimurium (ST)-existence vaccine in turkey poults. After vaccination at day time of hatch the ability of the vaccine to stimulate immune responses was evaluated. Several immune parameters were examined after vaccination, including the measurement of IgG serum antibody titers by ELISA until 3?weeks of age, but no increase in antibody titers in vaccinated turkey poults could be observed [7]. However, in several studies concerning vaccines and additional vaccines RIP2 kinase inhibitor 2 in turkeys antibody production began at three to 4?weeks of age independently from your timepoint of vaccination [8C10]. Also, only few parrots were used in our earlier study. The objective of the present study was consequently to determine if antibody production could be recognized, if certain guidelines were changed. This included that vaccinated parrots were observed for a longer period of time, that a greater quantity of parrots was used and that parrots received booster vaccinations. To our knowledge antibody production after vaccination has not been analyzed before over such a long period of time in turkeys. Additionally we examined the invasiveness of the vaccine strains and their excretion after vaccination at the 1st?day of existence and after the respective booster vaccinations. Main text Materials and methods Experimental design, sample collection and preparationCommercially available fattening turkeys, type BUT Big 6 (Moorgut Kartzfehn von Kameke GmbH & Co. KG, Germany) were utilized for the experiments. Multiple regular bacteriological control of the parent flock and serological examination of poults proved the free status of the parrots. On the day of hatching, woman turkey poults were randomly divided into two groups of 76 parrots each. One group (Additional file 1: Table S1) was vaccinated with the bivalent SE/ST vaccine at the 1st?day of existence with booster vaccinations at FANCF 6, 16 and 23?weeks of age, whereas the other group remained untreated while control group. Generally on days 3, 7, 14 and 21 after each vaccination and additionally on day time 5 after the 1st vaccination five animals were sacrificed by exsanguination after they had been stupefied by by hand applied blunt push.